食品科学 ›› 2009, Vol. 30 ›› Issue (18 ): 131-134.doi: 10.7506/spkx1002-6300-200918026

• 工艺技术 • 上一篇    下一篇

响应曲面法优化香薷多糖的提取工艺

李景恩,聂少平* ,杨 超,邱增辉,谢明勇,李 昌,万 茵   

  1. 南昌大学 食品科学与技术国家重点实验室
  • 收稿日期:2009-04-21 出版日期:2009-09-15 发布日期:2010-12-29
  • 通讯作者: 聂少平 E-mail:spnie@ncu.edu.cn
  • 基金资助:

    国家大学生创新性实验项目(081040306);食品科学与技术国家重点实验室目标导向项目(SKLF-MB-200806)

Application of Response Surface Methodology to Optimize Extraction Conditions of Polysaccharides from Herba Moslae

LI Jing-en,NIE Shao-ping*,YANG Chao,QIU Zeng-hui,XIE Ming-yong,LI Chang,WAN Yin   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Received:2009-04-21 Online:2009-09-15 Published:2010-12-29
  • Contact: NIE Shao-ping E-mail:spnie@ncu.edu.cn

摘要:

为优化热水浸提香薷多糖工艺,在单因素试验基础上,采用响应曲面法对影响香薷多糖提取率的三个主要因素即提取温度、提取时间和液固比进行优化。利用SAS V8.0 软件对香薷多糖提取率的二次多项数学模型分析表明:在提取温度87.0℃、提取时间4.0h、液固比15.4:1(ml/g)时,香薷多糖提取率较高,最佳提取率预测值为3.38%,与实测值3.41% 基本相符。

关键词: 香薷多糖, 提取, 响应曲面法

Abstract:

The hot water extraction procedure of Herba Moslae polysaccharides was studied. On the basis of single-factor experiments, a three-factor, three-level Box-Behnken design combining with response surface methodology (RSM) was employed to optimize three crucial extraction conditions for maximizing yield of polysaccharides from Herba Moslae. A regression model was established for yield of polysaccharides as a function of the three extraction conditions. The analysis for this model by SAS V8.0 software indicated that the optimal extraction temperature, time and ratio of water to material were 87.0 ℃, 4.0 h and 15.4:1 (ml/g), respectively. Under these conditions, the predicted value of yield of polysaccharides from Herba moslae was 3.38%, in basic agreement with the actual value of 3.41%.

Key words: polysaccharides from Herba Moslae, extraction, response surface methodology

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